Recombinant Human Dickkopf-Related Protein 1/DKK-1 (C-Avi-6His) Biotinylated
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH034043-20, PKSH034043-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: DKK1; Dkk-1
Target Symptom: Dickkopf-related protein 1; Dickkopf-1; Dkk-1
Research Area: Cardiovascular; Developmental Biology; immunology; Stem cells
Conjugation: Biotinylated
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-Avi-6His
UNIProt ID: O94907
Accession: O94907
Background: Dickkopf-related protein 1(DKK-1), is a member of the dickkopf family. DKK1 secreted proteins with two cysteine-rich domains separated by a linker region. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Sequence: Thr32-His266
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 28.4 kDa
Observed MW: 35-50 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only