Recombinant Human DR6/TNFRSF21 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031800-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: DR6; TNFRSF21
Target Symptom: Tumor Necrosis Factor Receptor Superfamily Member 21; Death Receptor 6; CD358; TNFRSF21; DR6
Research Area: Cell biology
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
Accession: NP_055267.1
Background: TNFRSF21 (death receptor-6; DR6) is an orphan TNF receptor superfamily member and belongs to a subgroup of receptors called death receptors. This type I transmembrane receptor possesses four extracellular cysteine-rich motifs and a cytoplasmic death domain. DR6 is an extensively posttranslationally modified transmembrane protein and that N- and O-glycosylations of amino acids in its extracellular part. DR6 interacts with the adaptor protein TRADD and mediates signal transduction through its death domain; and expression of DR6 in mammalian cells induces activation of both NF-kappaB and JNK and cell apoptosis. DR6 knockout mice have enhanced CD4+ T cell proliferation and Th2 cytokine production; suggested that DR6 serves as an important regulatory molecule in T-helper cell activation; and is involved in inflammation and immune regulation. DR6 is expressed ubiquitously with high expression in lymphoid organs; heart; brain and pancreas. Some tumor cells overexpress DR6; typically in conjunction with elevated anti-apoptosis molecules. DR6 may also be involved in tumor cell survival and immune evasion; which is subject to future investigations.
Activity: 1. Immobilized recombinant human DR6-his at 10 μg/mL (100 μl/well) can bind biotinylated human APP-Fc with a linear range of 0.0125-0.4 μg/mL.
Sequence: Met 1-Leu 350
Purity: > 96 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 35 kDa
Observed MW: 50-60 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only