Recombinant Human E2F2 protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100410-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: E2F2
Target Synonym: Transcription factor E2F2; E2F2;
UNIProt ID: Q14209
Research Areas: Epigenetics and Nuclear signaling
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: Q14209
Background: E2F-2 (viral E2-associated factor 2) is a 50-55 kDa member of the E2F/DP family of transcription factors. It is principally expressed by placenta, and forms a DNA-activating E2F heterodimeric complex with DP-1 or -2. This complex, when active, promotes cell cycle progression. In quiescent cells, association with the retinoblastoma-tumor suppressor gene product termed pRB suppresses its activity. Human E2F-2 is 437 amino acids (aa) in length and contains a CDK2 binding region (aa 65-105), a DNA-binding domain (aa 107-196), a dimerization segment (aa 197-289), a transactivation region (aa 359-437), and a pRB binding domain (aa 410-427). There are two potential alternate start sites at Met197 and Met342, and one splice variant that shows a two aa substitution for aa 349-437.
Sequence: Ala 65-Asn 437
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 40.92 kDa
Observed MW: 55 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only