Recombinant Human EGFR/ErbB1 Protein (aa 25-645, Fc Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032398-10, PKSH032398-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: EGFR; ErbB1
Target Symptom: Epidermal growth factor receptor; EGFR; Proto-oncogene c-ErbB-1; Receptortyrosine-protein kinase erbB-1; EGFR
Research Area: Signal Transduction; Cancer;
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc
UNIProt ID: P00533
Accession: P00533
Background: EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK; PI3 kinase-AKT; PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16; activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling.
Activity: Immobilized Human EGF(Cat: PKSH033687) at 10μg/ml(100 μl/well) can bind Human EGFR-Fc.
Sequence: Leu25-Ser645
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 95.7 kDa
Observed MW: 130 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only