Recombinant Human EphB1/EPHT2 Protein (aa 564-984, His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033688-10, PKSH033688-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: EphB1; EPHT2
Target Symptom: Ephrin Type-B Receptor 1; ELK; EPH Tyrosine Kinase 2; EPH-Like Kinase 6; EK6; hEK6; Neuronally-Expressed EPH; Related Tyrosine Kinase; NET; Tyrosine-Protein Kinase Receptor EPH-2; EPHB1; ELK; EPHT2; HEK6
Research Area: Signal Transduction; Cardiovascular; Neuroscience; Cancer; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
UNIProt ID: P54762
Accession: P54762
Background: Ephrin Type-B Receptor 1 (EPHB1) is a single-pass type I membrane protein that belongs to the Ephrin-B family of receptor tyrosine kinases that is involved in embryonic nervous and vascular system development. EPHB1/EPHT2 contains two fibronectin type-III domains; one protein kinase domain and one SAM (sterile α motif) domain. EPHB1 could stimulate fibroblast motility on extracellular matrix in a kinase-dependent manner; which also correlated with its association with Grb7; an adaptor molecule implicated in the regulation of cell migration. It binds to ephrin-B1; ephrin-B2 and ephrin-B3. EPHB1 plays an important roles in diverse biological processes including nervous system development; angiogenesis; and neural synapsis formation and maturation and may be involved in cell-cell interactions in the nervous system.
Activity: Immobilized Human EphB1-His at 10μg/ml(100 μl/well) can bind Mouse EFNB2-Fc(Cat: PKSM041012). The ED50 of Human EphB1-His is 53. 1 ug/ml.
Sequence: Ser564-Ala984
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris; 150mM NaCl; pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 48.8 kDa
Observed MW: 49 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only