Recombinant Human EphB2 Protein (Fc Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032012-10, PKSH032012-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: EphB2
Target Symptom: CAPB; DRT; EK5; EPHT3; ERK; Hek5; PCBC; Tyro5; EPHB2; Ephrin type-B receptor 2
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc
UNIProt ID: P29323
Accession: P29323-3
Background: Ephrin type-B receptor 2(EPHB2) belongs to the protein kinase superfamily and Ephrin receptor subfamily. EPHB2 contains 1 Eph LBD domain; 2 fibronectin type-III domains; 1 protein kinase domain and 1 SAM domain. Ephrin receptors and their ligands; the ephrins; mediate numerous developmental processes; particularly in the nervous system. Based on their structures and sequence relationships; ephrins are divided into the ephrin-A (EFNA) class; which are anchored to the membrane by a glycosylphosphatidylinositol linkage; and the ephrin-B (EFNB) class; which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family.
Activity: Immobilized Human EphB2-Fc at 2μg/ml(100 μl/well) can bind Human EFNB2-His(Cat: PKSH032395).
Sequence: Val19-Ser482
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 78.5 kDa
Observed MW: 120 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only