Recombinant Human Ephrin-A1/EFNA1 Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032388-10, PKSH032388-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Ephrin-A1; EFNA1
Target Symptom: Ephrin-A1; EPH-Related Receptor Tyrosine Kinase Ligand 1; LERK-1; Immediate Early Response Protein B61; Tumor Necrosis Factor Alpha-Induced Protein 4; TNF Alpha-Induced Protein 4; EFNA1; EPLG1; LERK1; TNFAIP4
Research Area: Signal Transduction; Cardiovascular; Neuroscience;
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-6His
UNIProt ID: P20827
Accession: P20827
Background: Ephrin-A1 is a member of the A-type ephrin family of cell surface proteins that function as ligands for the A-type Eph receptor tyrosine kinase family. Ephrin-A1 can be induced by TNF and IL1B. Its expression levels can be down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells. Soluble Ephrin-A1 is necessary for the transformation of HeLa and SK-BR3 cells and participates in the relocalization of EPHA2 away from sites of cell-cell contact during transformation. Ephrin-A1 plays an important role in angiogenesis and tumor neovascularization.
Activity: Immobilized Human Ephrin-A1-His at 10μg/ml(100 μl/well) can bind Human EphA2-Fc(Cat: PKSH032384). The ED50 of Human Ephrin-A1-His is 12.43ug/ml.
Sequence: Asp19-Ser182
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl; pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 20.4 kDa
Observed MW: 24-28 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only