Recombinant Human Ephrin-A4/EFNA4 Protein (Fc & His Tag)(Active)
Size: 50μg
Catalogue Number: PKSH032392-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Ephrin-A4; EFNA4
Target Symptom: Ephrin-A4; EPH-Related Receptor Tyrosine Kinase Ligand 4; LERK-4; EFNA4; EPLG4; LERK4
Research Area: Cardiovascular; Neuroscience; Developmental Biology
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc-6His
UNIProt ID: P52798
Accession: P52798
Background: Ephrin-A4 is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence; which most likely corresponds to the receptor binding domain. Ephrin-A4 consists of approximately 125 amino acids and includes four invariant cysteines; It has been shown to bind EphA2; EphA3; EphA4; EphA5; EphA6; EphA7; and EphB1. Ephrin-A4 binds promiscuously Eph receptors residing on adjacent cells; leading to contact-dependent bidirectional signaling into neighboring cells. It may play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils.
Activity: Immobilized Human EphA7-His(Cat: PKSH033689) at 2μg/ml(100 μl/well) can bind Human EFNA4-Fc-6His. The ED50 of Human EFNA4-Fc-6His is 1. 5190 ug/ml.
Sequence: Leu26-Gly171
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 44.3 kDa
Observed MW: 45 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only