Recombinant Human Ephrin-B2/EFNB2 Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSH032395-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Ephrin-B2; EFNB2
Target Symptom: Ephrin-B2; EPH-Related Receptor Tyrosine Kinase Ligand 5; LERK-5; HTK Ligand; HTK-L; EFNB2; EPLG5; HTKL; LERK5
Research Area: Cell biology; Cardiovascular; Neuroscience; Cancer
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-6His
UNIProt ID: P52799
Accession: P52799
Background: Ephrin-B2 is a type I transmembrane protein and belongs the Ephrin family. It binds to the receptor tyrosine kinases, such as EPHA4, EPHB4 and EPHA3. Ephrin-B2 has been implicated in mediating developmental events, especially in the nervous system, erythropoiesis and tumour metastasis. Ligation of Ephrin-B2 with complementary EphB receptors on adjacent cells results in a combination of forward (EphB receptors) and reverse (Ephrin-B2) signalling, which is central to tissue development and remodelling functions. In addition, Ephrin-B2 may play a role in constraining the orientation of longitudinally projecting axons.
Activity: Immobilized Human EphB2-Fc(Cat: PKSH032012) at 10μg/ml(100 μl/well) can bind Human AQZ9-His. The ED50 of Human AQZ9-His is 1. 13ug/ml.
Sequence: Ile28-Ala229
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 23.2 kDa
Observed MW: 35 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only