Recombinant Human ERN1/IRE1 Protein (aa 465-977, His & GST Tag)(Active)
Size: 50μg
Catalogue Number: PKSH030346-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: ERN1; IRE1
Target Symptom: hIRE1p; IRE1; IRE1a; IRE1P
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion Tag: N-His & GST
UNIProt ID: O75460
Accession: O75460-1
Background: The encoded protein contains two functional catalytic domains; a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins; regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
Activity: 1. Kinase activity untested 2. The specific activity was determined to be 5 nmol/min/mg using MBP as substrate
Sequence: Pro 465-Leu 977
Purity: > 80 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 86 kDa
Observed MW: 90 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only