Recombinant Human ERP57/PDIA3 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032955-10, PKSH032955-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: ERP57; PDIA3
Target Synonym: PDIA3 protein; Protein disulfide-isomerase A3; 58 kDa glucose-regulated protein; Disulfide isomerase ER-60; ER protein 57
UNIProt ID: P30101
Research Areas: Signal Transduction;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P30101
Background: PDIA3 protein is also known as Protein disulfide-isomerase A3. It is a protein that in humans is encoded by the PDIA3 gene.PDIA3 is an enzyme that belongs to the endoplasmic reticulum and interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. PDIA3 interacts with thiazide-sensitive sodium-chloride cotransporter in the kidney and is induced by glucose deprivation. PDIA3 is part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is important for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface.
Sequence: Ser25-Leu505
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10%Glycerol, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 55.3 kDa
Observed MW: 60 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only