Recombinant Human FGF-2/FGFb Protein (aa 134-288)(Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH032438-20, PKSH032438-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: FGF-2; FGFb
Target Symptom: Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; Bfgf; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB
Research Area: Signal Transduction; Cardiovascular; Neuroscience; Cancer; Developmental Biology; Stem cells
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion Tag: None
UNIProt ID: P09038
Accession: P09038
Background: Fibroblast growth factor 2(FGF2) is a secreted protein and belongs to the heparin-binding growth factors family. FGF2 is produced by epithelial; tumor and other cell types. It involved in developmental processes and regulates differentiation; proliferation; and migration; FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF2 has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase.
Activity: Measured in a cell proliferation assay using Balb/3T3 mouse fibroblast cells. The ED50 for this effect is 0.1-0.6 ng/ml
Sequence: Met 134-Ser288
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 17.2 kDa
Observed MW: 17 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only