Recombinant Human FGF-2/FGFb Protein (aa 143-288)(Active)
Size: 50μg
Catalogue Number: PKSH032439-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: FGF-2; FGFb
Target Symptom: Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
Research Area: Signal Transduction; Cardiovascular; Neuroscience; Cancer; Developmental Biology; Stem cells
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion Tag: None
UNIProt ID: P09038
Accession: P09038
Background: FGF-basic is a members of the Fibroblast Growth Factors (FGFs) family.The family constitutes a large family of proteins involved in many aspects of development including cell proliferation; growth; and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis; cell growth; pattern formation; embryonic development; metabolic regulation; cell migration; neurotrophic effects; and tissue repair. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain; pituitary; kidney; retina; bone; testis; adrenal gland liver; monocytes; epithelial cells and endothelial cells.
Activity: Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.3-2.0 ng/ml.
Sequence: Pro143-Ser288
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 0.02% Tween80, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
Calculated MW: 16.3 kDa
Observed MW: 17 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only