Recombinant Human Fibronectin/FN Protein (His & Avi Tag)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH033677-20, PKSH033677-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Fibronectin; FN
Target Symptom: Fibronectin; FN1; CIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF
Research Area: Signal Transduction; Cardiovascular; Cancer; Developmental Biology; Stem cells
Conjugation: Biotinylated
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His-Avi
UNIProt ID: P02751
Accession: P02751
Background: Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Similar to integrins; fibronectin binds extracellular matrix components such as collagen; fibrin; and heparan sulfate proteoglycans. Fibronectin plays a major role in cell adhesion; growth; migration; and differentiation; and it is important for processes such as wound healing and embryonic development. Altered fibronectin expression; degradation; and organization has been associated with a number of pathologies; including cancer and fibrosis. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer; named superfibronectin; exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth; angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Sequence: Glu5-Thr95
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 13.4 kDa
Observed MW: 13 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only