Recombinant Human Gastric Lipase/LIPF Protein (Human Cells, His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032480-10, PKSH032480-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Gastric Lipase; LIPF
Target Synonym: HGL; HLAL; Gastric Triacylglycerol Lipase; GL; Gastric Lipase; LIPF
UNIProt ID: P07098
Research Areas: Signal Transduction; Cardiovascular; Cancer; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P07098
Background: Gastric Triacylglycerol Lipase (LIPF) belongs to the AB hydrolase superfamily. LIPF is an important lipase during the digestion of dietary lipids in cystic fibrosis. LIPF is involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. LIPF is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. LIPF acts distinct roles in neutral lipid metabolism.
Sequence: Leu20-Lys398
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 100mM glycine, 10% glycerol, pH 7.3.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 44.2 kDa
Observed MW: 50 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only