Recombinant Human Glypican 5/GPC5 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031890-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Glypican 5; GPC5
Target Symptom: GPC5; bA93M14.1; glypican proteoglycan 5; glypican-5
Research Area: Signal Transduction;
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Application: ELISA
Fusion Tag: C-His
Accession: NP_004457.1
Background: Glypican-5 (GPC5), is a cell membrane protein which belongs to the glypican family. The glypicans compose a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans that may play a role in the control of cell division and growth regulation. So far, six members (Glypican-1/GPC1, Glypican-2/GPC2, Glypican-3/GPC3, Glypican-4/GPC4, Glypican-5/GPC5, Glypican-6/GPC6) of this family are known in vertebrates. In adult, Glypican-5 is primarily expressed in the brain. It is also detected in fetal brain, lung and liver. Glypican-5 enhances the intracellular signaling of FGF2 and HGF. It alters the cellular distribution of FGF2. The properties of Glypican-5 make it an attractive target for therapeutic intervention in rhabdomyosarcomas and other tumors that amplify and/or overexpress its gene. Glypican-5 is over-expressed in lymphoma cell lines that had shown amplification. It is a likely target for amplification, and that over-expression of GPC5 may contribute to development and/or progression of lymphomas and other tumors.
Activity: Immobilized human GPC5 at 5 μg/ml (100 μl/well) can bind human bFGF with a linear ranger of 0.156-2. 5 ng/ml.
Sequence: Met 1-Thr 554
Purity: > 92 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 60.5 kDa
Observed MW: 60.5 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only