Recombinant Human GRB2 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032512-10, PKSH032512-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: GRB2
Target Synonym: Growth Factor Receptor-Bound Protein 2; Adapter Protein GRB2; Protein Ash; SH2/SH3 Adapter GRB2; GRB2; ASH
UNIProt ID: P62993
Research Areas: Signal Transduction; Cancer;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
Accession: P62993
Background: As an adaptor protein, Growth Factor Receptor-Bound Protein 2 (GRB2) is involved in siganl transduction and consists of a central SH2 domain flanked by two SH3 domains. GRB2 associates with activated Tyr-phosphorylated EGF receptor/EGFR and PDGF receptors via its SH2 domain, stimulating GTP binding to Ras, which in turn activates MAPK and other signaling pathway.It also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK. probably via the concerted action of both its SH2 and SH3 domains.
Sequence: Met 1-Val217
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 26.3 kDa
Observed MW: 18-30 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only