Recombinant Human Histone H4/HIST2H4A Protein
Size: 100μg
Catalogue Number: PKSH031359-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Histone H4, HIST2H4A
Target Synonym: FO108, H4, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/J, H4/K, H4/M, H4/N, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, HIST1H4A, HIST1H4B H4/I, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A
Research Areas: epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: None
Accession: NP_003539.1
Background: Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.
Sequence: Met 1-Gly103
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 2 mM β-ME in dd H2O, pH 6.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 11.4 kDa
Observed MW: 12 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only