Recombinant Human HVEM/TNFRSF14 Protein (His Tag)
Size: 50μg
Catalogue Number: PKSH033657-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: HVEM; TNFRSF14
Target Symptom: Tumor Necrosis Factor Receptor Superfamily Member 14; Herpes Virus Entry Mediator A; Herpesvirus Entry Mediator A; HveA; Tumor Necrosis Factor Receptor-Like 2; TR2; CD270; TNFRSF14; HVEA; HVEM
Research Area: Signal Transduction; Cancer; immunology;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-His
UNIProt ID: Q92956
Accession: Q92956
Background: Herpesvirus entry mediator (HVEM) is a type I membrane protein in the TNF receptor superfamily; and it can both promote and inhibit T cell activity. HVEM is highly expressed on naive CD4+ T cells; CD8+ T memory cells; regulatory T cells; dendritic cells; monocytes; and neutrophils. It functions as a receptor for BTLA; CD160; LIGHT/TNFSF14; and Lymphotoxin-alpha. Ligation of HVEM by LIGHT triggers T cell; monocyte; and neutrophil activation and contributes to Th1 inflammation and cardiac allograft rejection. In contrast; HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation and dampens intestinal inflammation. HVEM enhances the development of CD8+ T cell memory and Treg function. It is additionally expressed on intestinal epithelial cells; where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense. The herpesvirus envelope glycoprotein gD; which binds HVEM to initiate membrane fusion; can antagonize both BTLA and LIGHT binding.
Sequence: Leu39-Val202
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, PH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 18.38 kDa
Observed MW: 28 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only