Recombinant Human ICOS Ligand/ICOSL Protein (Fc Tag)
Size: 100μg
Catalogue Number: PKSH031086-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: ICOS Ligand, ICOSL
Target Synonym: ICOS Ligand, B7 Homolog 2, B7-H2, B7-Like Protein Gl50, B7-Related Protein 1, B7RP-1, CD275, ICOSLG, B7H2, B7RP1, ICOSL, KIAA0653
UNIProt ID: O75144
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-hFc
Accession: O75144-1
Background: Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.
Sequence: Met 1-Ser258
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 53.7 kDa
Observed MW: 66-76 and 33 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only