Recombinant Human IDH1 Protein (R132H, C-8His)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032660-10, PKSH032660-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: IDH1
Target Synonym: Isocitrate Dehydrogenase [NADP] Cytoplasmic; IDH; Cytosolic NADP-Isocitrate Dehydrogenase; IDP; NADP(+)-Specific ICDH; Oxalosuccinate Decarboxylase; IDH1; PICD
UNIProt ID: O75874
Research Areas: Signal Transduction; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-8His
Accession: O75874
Background: Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 exists as a homodimer; binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma. IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions; such as the conversion of 2; 4-dienoyl-CoAs to 3-enoyl-CoAs; as well as in peroxisomal reactions that consume 2-oxoglutarate; namely the α-hydroxylation of phytanic acid.
Sequence: Met1-Leu414(Arg132His)
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 50mM Tris, 200mM NaCl, 10% glycerol, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 48.1 kDa
Observed MW: 40-50 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only