Recombinant Human IDO1/IDO Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032584-10, PKSH032584-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: IDO1; IDO
Target Symptom: Indole 2; 3-dioxygenase; Indoleamine 2; 3-dioxygenase 1; IDO-1; IDO1; IDO; INDO
Research Area: Signal Transduction; Cardiovascular; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Application: Enzyme
Fusion Tag: N-6His
UNIProt ID: P14902
Accession: P14902
Background: Indoleamine 2, 3-dioxygenase (IDO) is a heme enzyme that initiates the oxidative degradation of the least abundant, essential amino acid, l-tryptophan, along the kynurenine pathway. This protein is normally expressed in the dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. IDO activity is associated with immunosuppression and immune attenuation. Several studies showed that IDO can contribute to immune escape when expressed directly in tumor cells or when expressed in immunosuppressive antigen presenting cells such as tolerogenic dendritic cells or tumor associated macrophages. IDO also is a promising therapeutic target for the treatment of cancer, chronic viral infections, and other diseases characterized by pathological immune suppression.
Activity: Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is 5166. 667 pmol/min/µg pmol/min/μg.
Sequence: Met 1-Gly403
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Sodium Acetate, 150mM NaCl and 20% Glycerol, pH4.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 46.8 kDa
Observed MW: 40-50 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only