Recombinant Human IL-2RB/CD122 Protein (Fc Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032572-10, PKSH032572-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: IL-2RB; CD122
Target Symptom: Interleukin-2 receptor subunit beta; IL2RB; IL-2 receptor subunit beta; IL-2R subunit beta; High affinity IL-2 receptor subunit beta; CD122
Research Area: Signal Transduction; immunology; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc
UNIProt ID: P14784
Accession: P14784
Background: Human IL-2RB; also known asinterleukin-2 receptor subunit beta; is the receptor for interleukin-2. IL2 receptor complex is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2 receptor complex has three forms with respect to ability to bind IL2. IL-2RB is belonged to a type I membrane protein; and has a 26 residue signal peptide; a 214 residue extracellular region; a 25 residue transmembrane region and a 286 residue cytoplasmic domain. IL-2RB is the subunit critical for receptor-mediated signaling via physically or functionally coupling to other signaling molecules; such as the Jak-STAT and Src-family protein tyrosine kinase although it lacks apparent catalytic motifs.
Activity: Immobilized Human IL-2(Cat: PKSH032629) at 10μg/ml(100 μl/well) can bind Human IL-2RB-Fc. The ED50 of Human IL-2RB-Fc is 1. 37 ug/mL
Sequence: Ala27-Asp239
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 51.7 kDa
Observed MW: 60-80 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only