Recombinant Human IL6ST/CD130 Protein (His & Fc Tag)(Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH031330-20, PKSH031330-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: IL6ST; CD130
Target Symptom: CD130; CDW130; GP130; IL-6RB
Research Area: Signal Transduction; Cell biology; Cardiovascular; Cancer; immunology;
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His & Fc
Accession: NP_002175.2
Background: Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.
Activity: 1. Measured by its ability to bind human IL11Ra in a functional ELISA.
2. Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.2-0.8 μg/ml in the presence I'n tho pesence of 50 nglml. rcombnant human LsR and 100 ng/mL recombinant human IL-6.
Sequence: Met 1-Ile 618
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 96 kDa
Observed MW: 125-140 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only