Recombinant Human Interferon γ/IFNG Protein
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH032612-20, PKSH032612-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Interferon γ; IFNG
Target Symptom: Interferon Gamma; IFN-Gamma; Immune Interferon; IFNG
Research Area: Cancer; immunology; Stem cells
Target Species: Human Expression
Host: E.coli
Application: Cell culture Fusion
Tag: C-His
UNIProt ID: P01579
Accession: P01579 Background: IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them; leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens; mitogens; or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2; FGF-basic; and EGF.
Activity: Measure by its ability to induce cytotoxicity in HT29 cells. The ED50 for this effect is < 0.5 ng/mL The specific activity of recombinant human IFN gamma is approximately > 1. 3 x 107lU/mg. Sequence: Gln24-Gln166
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from 1×PBS, PH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
Calculated MW: 16.8 kDa
Observed MW: 16 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only