Recombinant Human Interleukin-18/IL-18 Protein
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH033626-20, PKSH033626-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Interleukin-18; IL-18
Target Symptom: Interleukin-18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; GIF; IL-18; IL-1g; IL1F4; MGC12320; IL18
Research Area: Cancer; immunology; metabolism
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion Tag: C-His
UNIProt ID: Q14116
Accession: Q14116
Background: Interleukin-18 is a secreted protein and it belongs to the IL-1 family. IL-18 is a proinflammatory cytokine and produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL-4 dependent IgE and IgG1 production; and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine; and thus negatively regulate its biological activity. After stimulation with IL-18; natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells.
Activity: Measure by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is < 5 ng/mL.
Sequence: Tyr37-Asp193
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from 1×PBS, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
Calculated MW: 18.2 kDa
Observed MW: 17 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only