Recombinant Human Interleukin-21/IL-21 Protein (Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH031514-20, PKSH031514-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Interleukin-21; IL-21
Target Symptom: Interleukin-21; IL-21; Za11; IL21
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion Tag: None
UNIProt ID: Q9HBE4
Accession: Q9HBE4-1
Background: IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. It may promote the transition between innate and adaptive immunity. IL-21 has been tried as therapy for alleviating allergic responses. It can significantly decrease pro-inflammatory cytokines produced by T cells in addition to decreasing IgE levels in a mouse model for rhinitis (nasal passage inflammation)
Activity: Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.
Sequence: Gln30-Ser162
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4, 5 % Trehalose, 5 % Mannitol 0.01% Tween80
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 5 EU per mg of the protein as determined by the LAL method.
Calculated MW: 15.6 kDa
Observed MW: 17 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only