Recombinant Human Interleukin-3/IL-3 Protein (E.coli, His Tag)(Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH032639-20, PKSH032639-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Interleukin-3; IL-3
Target Symptom: Interleukin-3; IL-3; Hematopoietic Growth Factor; Mast Cell Growth Factor; MCGF; Multipotential Colony-Stimulating Factor; P-Cell-Stimulating Factor; IL3
Research Area: immunology
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion Tag: C-His
UNIProt ID: P08700
Accession: P08700
Background: Interleukin-3 (IL-3) is a potent growth promoting cytokine. IL-3 can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological function through binding to specific cell surface receptors. The amino acid sequences of this protein among different species share relatively low identity and its activity is highly species-specific. IL-3 has also been shown to possess neurotrophic activity; and is thought to be associated with neurologic disorders.
Activity: Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.15 ng/mL. The specifc activity of recombinant human IL-3 is approximately > 1. 2 x 106lU/mg.
Sequence: Ala20-Phe152
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from 1×PBS, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
Calculated MW: 16.1 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only