Recombinant Human Interleukin-7/IL-7 Protein (Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH033610-20, PKSH033610-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Interleukin-7; IL-7
Target Symptom: Interleukin-7; IL-7; IL7
Research Area: immunology
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion Tag: C-His
UNIProt ID: P13232
Accession: P13232
Background: Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65% sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT; which regulate lymphocyte survival; glucose uptake; proliferation; and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.
Activity: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC). The ED50 for this effect is 0.8ng/ml. The specific activity of recombinant human IL-7 is > 7 x10^8 lU/mg.
Sequence: Asp26-His177
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from filtered solution of PBS, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
Calculated MW: 17.5 kDa
Observed MW: 18 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only