Recombinant Human JAM-A/F11R Protein (Fc Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031772-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: JAM-A; F11R
Target Symptom: Junctional Adhesion Molecule A; JAM-A; Junctional Adhesion Molecule 1; JAM-1; Platelet F11 Receptor; Platelet Adhesion Molecule 1; PAM-1; CD321; F11R; JAM1; JCAM; JAMA; JCAM; KAT
Research Area: Signal Transduction; Cardiovascular;
Target Species: Human
Expression Host: HEK293 Cells
Application: Cell culture; ELISA
Fusion Tag: C-hFc
Accession: NP_058642.1
Background: Junctional adhesion molecule-A (JAM-A); also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321); is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells; as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1; involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily; JAM-A protein involves in platelet adhesion; secretion and aggregation; and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition; it may be a potential therapeutic target for breast cancer.
Activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 x 104cells/well are added to JAM-A-Fc coated plates (2. 5μg/mL, 100 μL/well)in the presence of 20 ng/mL PMA, approximately 30-40% will adhere after 30 minutes at 37℃.
Sequence: Met 1-Ala 242
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 50 kDa
Observed MW: 61 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only