Recombinant Human KLRB1/CD161 Protein (Fc Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032676-10, PKSH032676-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: KLRB1; CD161
Target Synonym: Killer cell lectin-like receptor subfamily B member 1; KLRB1; CLEC5B; NKRP1A; CD161; Natural killer cell surface protein P1A; NKR-P1A; HNKR-P1a; C-type lectin domain family 5 member B; KLRB1
UNIProt ID: Q12918
Research Areas: immunology
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-Fc
Accession: Q12918
Background: Killer cell lectin-like receptor subfamily B, member 1(KLRB1) is a single-pass type II membrane protein which contains 1 C-type lectin domain. KLRB1 plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. It acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1, 3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
Sequence: Gln67-Ser225
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 45.6 kDa
Observed MW: 130 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only