Recombinant Human LAG3/CD223 Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033596-10, PKSH033596-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: LAG3; CD223
Target Symptom: Lymphocyte activation gene 3 protein; LAG3; LAG-3; Protein FDC; CD223; LAG-3
Research Area: immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
UNIProt ID: P18627
Accession: P18627
Background: Human Lymphocyte activation gene 3 protein( LAG3) is a member of immunoglobulin (Ig) superfamily. LAG3 contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. LAG3 is involved in lymphocyte activation and can bind to HLA class-II antigens. It is selectively expressed in activated T and NK cells. LAG3 has a negative regulatory function in T cells and acts as as a new marker of T cell induced B cell activation. As a soluble molecule; LAG3 activates antigen-presenting cells through MHC class II signaling. It can lead to increased antigen-specific T-cell responses in vivo. LAG-3 has higher affinity to MHC class II than CD4.
Activity: Immobilized Human LAG-3-His at 5μg/ml(100 μl/well) can bind Human FGL1-mFc(Cat: PKSH033679). The ED50 of Human LAG-3-His is 29 ng/ml.
Sequence: Leu23-Leu450
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS; 2mMEDTA; pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.2 kDa
Observed MW: 55-65 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only