Recombinant Human LAMP2/CD107b Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032684-10, PKSH032684-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: LAMP2; CD107b
Target Symptom: Lysosome-Associated Membrane Glycoprotein 2; LAMP-2; Lysosome-Associated Membrane Protein 2; CD107 Antigen-Like Family Member B; CD107b; LAMP2
Research Area: Signal Transduction; Tags & Cell Markers; Cardiovascular; Cancer; immunology; metabolism; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-6His
UNIProt ID: P13473
Accession: P13473
Background: Lysosomal Associated Membrane Protein 2 (LAMP2) is a major component of lysosomal membranes. LAMP2 is a transmembrane glycoprotein about 110kDa. Mature human LAMP2 consists of a 347 amino acid (aa) intralumenal domain, a 24 aa transmembrane segment, and a 35 aa cytoplasmic tail . The lumenal domain is organized into two heavily N-glycosylated regions. Alternate splicing generates a human LAMP2 isoform (LAMP2B) with a substituted juxtamembrane lumenal region, cytoplasmic tail and transmenmbrane segment.LAMP2 itself can cleavage lysosomal luminal domain and degradation lysosomal. In the help of chaperone HSC73, LAMP2 mediates the lysosomal uptake in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles.
Activity: Immobilized Human LGALS3(Cat: PKSH032474) at 1. 5μg/ml(100 μl/well) can bind Human LAMP2-His. The ED50 of Human LAMP2-His is 12.73 ug/ml.
Sequence: Leu29-Ile375
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 33.9 kDa
Observed MW: 78 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only