Recombinant Human LYPLA1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032043-10, PKSH032043-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: LYPLA1
Target Synonym: Acyl-Protein Thioesterase 1; APT-1; hAPT1; Lysophospholipase 1; Lysophospholipase I; LPL-I; LysoPLA I; LYPLA1; APT1; LPL1
UNIProt ID: O75608
Research Areas: Signal Transduction; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: O75608
Background: Acyl-Protein Thioesterase 1 (APT-1) is lysophospholipase that belongs to the AB hydrolase 2 family. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. APT-1 performs on biological membranes to regulate the multifunctional lysophospholipids. It hydrolyzes lysophosphatidylcholine in both monomeric and micellar forms. It hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS; in addition, it also has depalmitoylating activity and low lysophospholipase activity.
Sequence: Met 1-Asp230
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 10% Glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 26.8 kDa
Observed MW: 25 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only