Recombinant Human MECP2 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032751-10, PKSH032751-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: MECP2
Target Synonym: Methyl-CpG-binding protein 2; MECP2; MeCp-2 protein
UNIProt ID: P51608
Research Areas: Neuroscience; epigenetics and nuclear signal
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P51608
Background: The MeCP2 helps regulate gene activity (expression) by modifying chromatin, the complex of DNA and protein that packages DNA into chromosomes. The MeCP2 protein is present in cells throughout the body, although it is particularly abundant in brain cells.In the brain, the MeCP2 protein likely plays a role in maintaining connections (synapses) between neurons, where cell-to-cell communication occurs. The alternative splicing of proteins is critical for normal communication between neurons and may also be necessary for the function of other types of brain cells.
Sequence: Met 1-Ser486
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 53.5 kDa
Observed MW: 90 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only