Recombinant Human METAP2 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032750-10, PKSH032750-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: METAP2
Target Synonym: Methionine aminopeptidase 2; MAP 2; MetAP 2; p67; p67eIF2; Peptidase M; METAP2; MAP2
UNIProt ID: P50579
Research Areas: Signal Transduction; metabolism;
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Fusion Tag: N-6His
Accession: P50579
Background: Human Methionine Aminopeptidase 2 (METAP2, MAP2) is a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator
methionine residue from nascent peptides and are essential for cell growth. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. METAP2 plays an important role in the development of different types of cancer and has been a novel target for developing anti-cancer
drugs. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amno-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.
Sequence: Ala2-Tyr478
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 53.6 kDa
Observed MW: 72 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only