Recombinant Human MGMT Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032031-10, PKSH032031-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: MGMT
Target Synonym: Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNAmethyltransferase; O-6-methylguanine-DNA-alkyltransferase; MGMT
UNIProt ID: P16455
Research Areas: epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: P16455
Background: MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.
Sequence: Met 1-Asn207
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mMTris-HCl, 1mM EDTA, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 23.8 kDa
Observed MW: 25 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only