Recombinant Human MICA Protein (Fc Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032753-10, PKSH032753-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: MICA
Target Symptom: MHC Class I Polypeptide-Related Sequence A; MIC-A; MICA; PERB11.1
Research Area: immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc
UNIProt ID: Q29983
Accession: Q29983
Background: MHC class I polypeptide-related sequence A, also known as MIC-A, PERB11.1 and MICA, is a single-pass type I membrane protein which belongs to the MHC class I family of MIC subfamily. MICA contains one Ig-like C1-type domain and is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA is the ligand for NK cell activating receptor KLRK1/NKG2D. MICA seems to have no role in antigen presentation. MICA leads to cell lysis by binding to KLRK1.
Activity: Immobilized Human MICA-Fc at 2μg/ml(100 μl/well) can bind Human NKG2DL2-His(Cat: PKSH032816). The ED50 of Human MICA-Fc is 2. 29 ug/ml.
Sequence: Ala23-Glu308
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 59.9 kDa
Observed MW: 88 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only