Recombinant Human/Mouse/Rat BDNF Protein (Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH033808-20, PKSH033808-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: BDNF
Target Symptom: Brain-Derived Neurotrophic Factor; BDNF; Abrineurin
Research Area: Cardiovascular; Neuroscience; metabolism
Target Species: Human/Mouse/Rat Expression
Host: E.coli
Application: Cell culture
Fusion Tag: C-His
UNIProt ID: P23560
Accession: P23560
Background: Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF; NT-3 and NT-4; BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor; also known as p75). BDNF promotes the survival; growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson' s and Alzheimer' s disease.
Activity: Measure by its ability to induce proliferation in BaF3 cells transfected with TrkB. The ED50 for this effect is < 2 ng/mL.
Sequence: His129-Arg247
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM sodium citrate, 0.2M NaCl, pH 3.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
Calculated MW: 13 kDa
Observed MW: 14 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only