Recombinant Human Myeloperoxidase/MPO Protein (His Tag)
Sizes: 20μg, 100μg
Catalogue Numbers: PDMH100014-20, PDMH100014-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Myeloperoxidase; MPO
Target Synonym: Myeloperoxidase; MPO
UNIProt ID: P05164
Research Areas: Cardiovascular; Cancer; immunology; metabolism
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-10His
Accession: P05164
Background: Myeloperoxidase (MPO) is a heme-containing
enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that
catalyzes the hydrogen peroxide-dependent
conversion of chloride, bromide, and iodide to multiple reactive species. Post-translational
processing of MPO involves
the insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide. This results in a disulfide-linked
dimer composed of a
60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bond
between the heavy chains. Alternate splicing generates two additional isoforms of MPO, one with a 32 aa insertion in the light chain, and another with a deletion of
the signal sequence and part of the propeptide. Human and mouse MPO share 87% aa sequence identity. MPO activity results in protein nitrosylation and the
formation of 3-chlorotyrosine
and dityrosine crosslinks. MPO is also associated with a variety of other diseases, and inhibits vasodilation in inflammation by depleting the levels
of NO. Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells. MPO is stored in neutrophil azurophilic
granules. Upon cellular activation, it is deposited into pathogen-containing
phagosomes.
Sequence: Ala49-Ser745
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 80.3 kDa
Observed MW: 85-95 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only