Recombinant Human Nectin-3/PVRL3 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031388-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Nectin-3; PVRL3
Target Symptom: Poliovirus Receptor-Related Protein 3; CDw113; Nectin-3; CD113; PVRL3; PRR3; PVRR3
Research Area: Microbiology; immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
Accession: NP_056295.1
Background: Poliovirus receptor-related 3 (PVRL3), also known as Nectin-3 and CD113, is a member of the nectin family. PVRL3/Nectin-3 is an 83 kDa, type I transmembrane glycoprotein. Its precursor is 549 amino acids (aa) in length and contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa, and a cytoplasmic region of 124 aa. Nectin-3 has three splicing variants, nectin-3alpha (biggest), -3beta (middle), and -3gamma (smallest). It is predominantly expressed in testis and placenta as well as in various cell lines, including epithelial cell lines. PVRL3/Nectin-3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/Nectin-2 at Sertoli-spermatid junctions. PVRL3/Nectin-3 is thus involved in the formation of cell-cell junctions, including adherens junctions and synapses. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.
Activity: Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated Nectin-1 with a linear range of 6. 4-800 ng/ml. 2. Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind Nectin-1/ Fc chimera with a linear range of 0.156-5 ng/ml.
Sequence: Met 1-Asp 400
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 39.3 kDa
Observed MW: 65-75 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only