Recombinant Human NEF3 protein (GST tag)
Size: 100μg
Catalogue Numbers: PDEH100124-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: NEF3
Target Synonym: Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; NEFM; NEF3; NFM
UNIProt ID: P07197
Research Areas: Neuroscience
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-GST
Accession: P07197
Background: Human NF-M (neurofilament-medium length) is a 145-150 kDa phosphorylated glycoprotein that belongs to the type IV intermediate filament family. It is 915 amino acids in length and contains a 100 amino acid (aa) N-terminal globular region, a 320 aa alpha -helical rod, or central region, and a 500 aa globular tail. The rod region contains a series of heptad repeats that contribute to a coiled-coil interaction with an adjacent NF-M molecule, the first step in intermediate filament oligomerization. The C-terminus shows phosphorylation and O-linked glycosylation. Human NF-M aa 2-358, which includes most of the rod region, shares ~98% aa sequence identity with corresponding region of mouse, rat and canine NF-M.
Sequence: Ser 2-Ser 358
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 64.16 kDa
Observed MW: 60 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only