Recombinant Human NGAL/Lipocalin-2 Protein (His Tag, Human Cells)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032806-10, PKSH032806-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: NGAL; Lipocalin-2
Target Synonym: Neutrophil gelatinase-associated lipocalin; NGAL; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25; HNL; NGAL
UNIProt ID: P80188
Research Areas: Signal Transduction; Cardiovascular; Cancer; immunology; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P80188
Background: LCN2 is iron-trafficking protein involved in multiple processes such as apoptosis; innate immunity and renal development. LCN2 binds iron through association with 2; 5-dihydroxybenzoic acid (2; 5-DHBA); a siderophore that shares structural similarities with bacterial enterobactin; and delivers or removes iron from the cell; depending on the context. LCN2 is involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis; while iron-free form decreases intracellular iron levels; inducing expression of the proapoptotic protein BCL2L11/BIM; resulting in apoptosis. LCN2 is involved in innate immunity; possibly by sequestrating iron; leading to limit bacterial growth.
Sequence: Gln21-Gly198
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of PBS, 50% glycerol, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 21.6 kDa
Observed MW: 23 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only