Recombinant Human NME1/NDKA Protein (His Tag)
Size: 50μg
Catalogue Number: PKSH030357-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: NME1, NDKA
Target Synonym: Nucleoside Diphosphate Kinase A, NDK A, NDP Kinase A, Granzyme A-Activated DNase, GAAD, Metastasis Inhibition Factor nm23, Tumor Metastatic Process-Associated Protein, nm23-H1, NME1, NDPKA, NM23, AWD, GAAD, NB, NBS, NDKA, NDPK-A, NM23-H1
Research Areas: Cancer, epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: NP_000260.1
Background: NME1, also known as Nucleoside Diphosphate Kinase A (NDK-A), or NM23-H1, belongs to the NDK family. NM23-H1 is known to have a metastasis suppressive activity in many tumor cells. Recent studies have shown that the interacting proteins with NM23-H1 which mediate the cell proliferation, may act as modulators of the metastasis suppressor activity. The interacting proteins with NM23-H1 can be classified into 3 groups. The first group of proteins can be classified as upstream kinases of NM23-H1 such as CKI and Aurora-A/STK15. The second group of proteins acts as downstream effectors for the regulation of specific gene transcriptions, GTP-binding protein functions, and signal transduction in Erk signal cascade. The third group of proteins can be classified as bi-directionally influencing binding partners of NM23-H1. As a result, the interactions with NM23-H1 and binding partners have implications in the biochemical characterization involved in metastasis and tumorigenesis. NDKA is increased in human postmortem cerebrospinal fluid (CSF), a model of global brain insult, suggesting that measurement in CSF and, more importantly, in plasma may be useful as a biomarker of stroke. Additionally, NM23-H1 significantly reduces metastasis without effects on primary tumor size and was the first discovered metastasis suppressor gene.
Sequence: Ala 2-Glu 152
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: Please contact us for more information.
Calculated MW: 18 kDa
Observed MW: 21 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only