Recombinant Human NSE/ENO2 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033672-10, PKSH033672-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: NSE; ENO2
Target Symptom: Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE; ENO2
Research Area: Signal Transduction; Tags & Cell Markers; Neuroscience; Cancer; Developmental Biology; epigenetics and nuclear signal; metabolism; Stem cells
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
UNIProt ID: P09104
Accession: P09104
Background: Gamma-enolase; also known as Enolase 2; belongs to the enolase family. The alpha/alpha homodimer of ENO2 is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle; and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. During ontogenesis; there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells; and to the alpha/gamma heterodimer in nerve cells. Levels of ENO2 increase dramatically in cardiovascular accidents; cerebral trauma; brain tumors and Creutzfeldt-Jakob disease. ENO2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds to cultured neocortical neurons and promotes cell survival in a calcium-dependent manner.
Sequence: Met1-Leu434
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris; 100mM KCl; 5mM MgSO4; pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 49.4 kDa
Observed MW: 55 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only