Recombinant Human P4HB Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033399-10, PKSH033399-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: P4HB
Target Synonym: DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB; Protein Disulfide-Isomerase; PDI; Cellular Thyroid Hormone-Binding Protein; Prolyl 4-Hydroxylase Subunit Beta; p55
Research Areas: Signal Transduction; Tags & Cell Markers; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
Accession: NP_000909.2
Background: Protein Disulfide-Isomerase (P4HB) is an endoplasmic reticulum lumen protein that belongs to the protein disulfide isomerase family. P4HB contains two thioredoxin domains and catalyzes the formation, breakage, and rearrangement of -S-S- bonds in proteins. P4HB is involved in hydroxylation of prolyl residues in preprocollagen. P4HB has the ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner. P4HB plays a role in both the influx and efflux of S-nitrosothiol-bound nitric oxide.
Sequence: Asp18-Lys505
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 55.9 kDa
Observed MW: 68 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only