Recombinant Human PAPPA2/Pappalysin 2 Protein (His Tag)
Size: 50μg
Catalogue Number: PKSH031546-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PAPPA2, Pappalysin 2
Target Synonym: PAPP-A2, PAPP-E, PAPPE, PLAC3
Research Areas: Cell biology, Cardiovascular
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: NP_064714.2
Background: Pappalysin-2/PAPP-A2 is the second member of the pappalysin family of metzincin superfamily, of which PAPP-A is the first member. There is no homology between the prepro-peptides of PAPP-A and PAPP-A2, but 46% of the residues of mature PAPP-A are also present in mature PAPP-A2. PAPP-A specifically cleaves insulin-like growth factor-binding protein(IGFBP)-4, one of six known modulators of IGF-I and –II, whereas PAPP-A2 specifically cleaved IGFBP-5 at one site, between Ser-143 and Lys-144. In contrast to the cleavage of IGFBP-4 by PAPP-A that strictly requires the presence of IGF, the cleavage of IGFBP-5 by PAPP-A2 was IGF-independent. Recent data firmly establish PAPP-A and IGFBP-4 as an important functional pair in several systems. Because of its close relationship with PAPP-A, both structurally and functionally, PAPP-A2 is a likely candidate for IGFBP-5 proteinase in many tissues and conditioned media where IGFBP-5 proteolysis has been reported.
Sequence: Ser 234-Cys 1396
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01%, Tween-80
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 131 kDa
Observed MW: 170-180 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only