Recombinant Human PDCD4/H731 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032866-10, PKSH032866-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PDCD4; H731
Target Synonym: Programmed Cell Death Protein 4; Neoplastic Transformation Inhibitor Protein; Nuclear Antigen H731-Like; Protein 197/15a; PDCD4; H731
UNIProt ID: Q53EL6
Research Areas: Cell biology; Cancer; epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
Accession: Q53EL6
Background: Programmed Cell Death Protein 4 (PDCD4) is a member of the PDCD4 family. PDCD4 and EIF4A1 form a heterotrimer. One molecule of PDCD4 binds two molecules of EIF4A1. PDCD4 takes part in apoptosis via inhibiting translation initiation and cap-dependent translation.PDCD4 promotes colonic neoplastic transformation and tumor invasion. PDCD4 is an important target for microrna R-21 in breast cancer cells. Shortage of PDCD4 expression is associated with colorectal cancer. Overexpression of PDCD4 in carcinoid cells results in inhibition of cell proliferation.
Sequence: Lys212-Pro357
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 17.0 kDa
Observed MW: 17 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only