Recombinant Human PFKFB1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032459-10, PKSH032459-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PFKFB1
Target Synonym: 6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 1; 6PF-2-K/Fru-2; 6-P2ase liver isozyme; Fructose-2; 6-bisphosphatase; PFKFB1; F6PK; PFRX
UNIProt ID: P16118
Research Areas: Signal Transduction; Cardiovascular; Neuroscience; Cancer; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P16118
Background: 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 is an enzyme that in humans is encoded by the PFKFB1 gene. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2, 6-biphosphate using independent catalytic domains. It belongs to the phosphoglycerate mutase family. Fructose-2, 6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2, 6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis.
Sequence: Ser2-Tyr471
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 55.6 kDa
Observed MW: 60 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only