Recombinant Human PI3/Elafin Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032375-10, PKSH032375-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PI3; Elafin
Target Synonym: Elafin; Elastase-Specific Inhibitor; ESI; Peptidase Inhibitor 3; PI-3; Protease inhibitor WAP3; Skin-Derived Antileukoproteinase; SKALP; WAP Four-Disulfide Core Domain Protein 14; PI3; WAP3; WFDC14
UNIProt ID: P19957
Research Areas: Microbiology; Cell biology; Cancer; immunology
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P19957
Background: Elafin is a secreted protein. Elafin consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize Elafin covalently to specific sites on extracellular matrix proteins. It is shown that Elafin can be used as a biomarker for graft versus host disease of the skin. Elafin is expressed in the skin during wound healing through activation of the epidermal growth factor receptor. Elafin may prevent elastase-mediated tissue proteolysis.
Sequence: Ala23-Gln117
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 11.0 kDa
Observed MW: 13-15 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only